Improved resolution in fiber bundle inline holographic microscopy using multiple illumination sources.

Abstract

Recent work has shown that high-quality inline holographic microscopy images can be captured through fiber imaging bundles. Speckle patterns arising from modal interference within the bundle cores can be minimized by use of a partially-coherent optical source such as an LED delivered via a multimode fiber. This allows numerical refocusing of holograms from samples at working distances of up to approximately 1 mm from the fiber bundle before the finite coherence begins to degrade the lateral resolution. However, at short working distances the lateral resolution is limited not by coherence, but by sampling effects due to core-to-core spacing in the bundle. In this article we demonstrate that multiple shifted holograms can be combined to improve the resolution by a factor of two. The shifted holograms can be rapidly acquired by sequentially firing LEDs, which are each coupled to their own, mutually offset, illumination fiber. Following a one-time calibration, resolution-enhanced images are created in real-time at an equivalent net frame rate of up to 7.5 Hz. The resolution improvement is demonstrated quantitatively using a resolution target and qualitatively using mounted biological slides. At longer working distances, beyond 0.6 mm, the improvement is reduced as resolution becomes limited by the source spatial and temporal coherence.