Abstract
Supplementing commercial xylanase and cellulase with selected debranching enzymes only resulted in slight enhancement of the enzymatic hydrolysis of wheat bran autohydrolysis residues (WBAR) which was obtained at 160°C over a 30-min period of autohdyrolysis, while a blend of enzymes from Aspergillus niger and Eupenicillium parvum achieved synergistic efficacy in this context. Using an equal mixture blend of these enzymes at a 0.5% (w/w) enzyme loading dosage with the addition of ferulic acid esterase (1 U/g substrate), the obtained hydrolysis yields were desirable, including 84.98% of glucose, 84.74% of xylose, 80.24% of arabinose, and 80.86% of ferulic acid. Following further separation using an HP-20 resin, the final ferulic acid recovery levels were as high as 62.5% of the esterified ferulic acid present within the initial WBAR input. Together, these data suggest that a combination of autohydrolysis and enzymatic hydrolysis using crude enzyme blends can efficiently achieve wheat bran enzymatic saccharification and associated ferulic acid release.
Highlights
Wheat bran, which accounts for 25% of the weight of wheat grains, is a byproduct of the wheat production process
Wheat bran autohydrolysis residue (WBAR) derived from the pretreatment of de-starched wheat bran using hot water was subjected to further enzymatic hydrolysis in order to facilitate monosaccharide and Ferulic acid (FA) recovery through synergistic enzymatic activity using a blend of crude enzymatic extracts derived from Aspergillus niger and Eupenicillium parvum
de-starched WB (DSWB) used in the present study was primarily composed of cellulose, xylan, and arabinan, accounting for 19.1, 29.2, and 22.2% of the total weight, respectively, demonstrating the high levels of carbohydrates therein (Table 1)
Summary
Wheat bran, which accounts for 25% of the weight of wheat grains, is a byproduct of the wheat production process. Wheat bran autohydrolysis residue (WBAR) derived from the pretreatment of de-starched wheat bran using hot water was subjected to further enzymatic hydrolysis in order to facilitate monosaccharide and FA recovery through synergistic enzymatic activity using a blend of crude enzymatic extracts derived from Aspergillus niger and Eupenicillium parvum. This strategy offers potential value as a reliable approach to biomass hydrolysis for the preparation of fermentable sugars and natural FA. As far as our knowledge is concerned, there are fewer reports on the synergistic crude enzyme preparations from Eupenicillium and Aspergillus
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