Abstract
The recovery rates and times to detection of microorganisms isolated from two similar blood culture media, one containing saponin, were compared. A total of 2117 blood cultures were analysed in a prototype automated blood culture system. Significantly more gram-positive organisms (P<0.05) and gram-negative organisms (P<0.05), including Enterobacteriaceae (P<0.05) were recovered from the lytic medium. Average time to detection in the lytic medium was 15.8 h, compared to 22.7 h in the other medium (P<0.001). The improved recovery of microorganisms was most pronounced in blood samples obtained from patients being treated with antibiotics at the time of venesection. In vitro experiments on antibiotic affected bacteria confirmed the protective effect of saponin.
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More From: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
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