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Abstract
A spectrophotofluorometric method for the determination of isoproterenol hydrochloride in parenteral formulations is described. This method is quite simple, precise, and extremely specific for unchanged isoproterenol in decomposed formulations. The method depends on the initial oxidation of isoproterenol hydrochloride with iodine-potassium iodide solution in pH 4.0 (McIlvaine' s citrate-phosphate) buffer. The partially oxidized isoproterenol “chrome” derivative is cyclized to the fluorescent “lutin” derivative with strong alkali. The fluorescence is stabilized with alkaline ascorbate solution, which is subsequently determined. The influence of the various decomposition products of isoproterenol, such as isoproterenol sulfonic acid and isoproterenone, on the fluorometric procedure was investigated with concurrent determination of the same solutions by the USP XVIII method. The usefulness of the fluorometric procedure to analyze fresh as well as partially decomposed isoproterenol solutions, stabilized with other antioxidants, is evaluated. The inapplicability of the existing methods to analyze very dilute (1: 50,000) solutions of isoproterenol is also described. The specific advantages of the fluorometric procedure in the routine analysis of a large number of samples with speed and accuracy are discussed. Statistical evaluation of the fluorometric method is also reported.
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