Abstract
ATP synthase produces ATP, a major energy source for metabolic processes in organisms, from ADP and inorganic phosphate in cellular membranes. ATP synthase is known as a rotary motor, in which the c-subunit ring functions as a rotor. In this work, we have tried to develop a more general preparation procedure of thermophilic <TEX>$F_oc$</TEX>-ring (<TEX>$TF_oc$</TEX>-ring) for NMR measurements. The expression of <TEX>$TF_oF_1$</TEX> is easily affected by various experimental conditions such as temperature, shape and size of a flask, a volume of medium, and shaking rate of an incubator. Accordingly, we have tried to optimize the expression conditions of <TEX>$TF_oF_1$</TEX>. <TEX>$TF_oc$</TEX>-rings were purified from <TEX>$TF_oF_1$</TEX> according to a reported method. We modified purification procedures to improve purity and yield of <TEX>$TF_oc$</TEX>. On top of them, we found a new combination of detergents for the purification at anion-exchange column chromatography. To examine the effect of lipid environments on the structure, the <TEX>$TF_oc$</TEX>-rings were reconstituted into two kinds of lipid bilayers, namely, saturated and unsaturated lipid ones. Then, we have compared characteristics of the <TEX>$TF_oc$</TEX>-ring structures in these membranes with solid-state NMR.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
