Abstract

Pullulan production from Aureobasidium pullulans was explored to increase yield. Non-linear hybrid mathematical tools for optimization of process variables as well as the pullulan yield were analyzed. The one variable at a time (OVAT) approach was used to optimize the maximum pullulan yield of 35.16 ± 0.29 g/L. The tools predicted maximum pullulan yields of 39.4918 g/L (genetic algorithm coupled with artificial neural network (GA–ANN)) and 36.0788 g/L (GA coupled with adaptive network based fuzzy inference system (GA–ANFIS)). The best regression value (0.94799) of the Levenberg–Marquardt (LM) algorithm for ANN and the epoch error (6.1055 × 10−5) for GA–ANFIS point towards prediction precision and potentiality of data training models. The process parameters provided by both the tools corresponding to their predicted yield were revalidated by experiments. Among the two of them GA–ANFIS results were replicated with 98.82% accuracy. Thus GA–ANFIS predicted an optimum pullulan yield of 36.0788 g/L with a substrate concentration of 49.94 g/L, incubation period of 182.39 h, temperature of 27.41 °C, pH of 6.99, and agitation speed of 190.08 rpm.

Highlights

  • Pullulan is a biopolymer of high commercial importance and utility [1]

  • Through extensive study of the metabolic pathway of sucrose in pullulan fermentation, it was found that sucrose did not cleave into glucose and fructose in the medium [30,31]

  • Due to its forthcoming supplementary requirement, the fermentative production of pullulan by A. pullulans has been optimized by many statistical tools including RSM, Plackett–Burman, Box–Behnken design, etc

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Summary

Introduction

Pullulan is a biopolymer of high commercial importance and utility [1]. Pullulan which is mostly available in powdered form, can be formulated to thin films. These films have numerous applications in food, pharma, and healthcare industries. The most popular and commercially successful application of pullulan films is in Listerine® mouth freshener. Pullulan has received wider acceptance in the food sector, and due to its intensifying nature has become an accepted ingredient in soups, sauces, and beverages [2]. Pullulan can be used in pharmaceuticals, such as in coatings on pills and capsules, including sustained-release formulations [2]

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