Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene–divinylbenzene beads

Abstract

Two immobilized preparations from Thermomyces lanuginosus lipase (TLL) were compared in the synthesis of butyl butyrate. The commercial Lipozyme TL-IM, and TLL immobilized on styrene–divinylbenzene beads (MCI-TLL) were tested in the esterification reaction using n-hexane as solvent. The variables temperature (30–60°C), substrate molar ratio (1:1 to 5:1), added water (0–1%), and biocatalyst content (3–40%) were evaluated in terms of initial reaction rate for each biocatalyst. SDS–PAGE analysis revealed that MCI-TLL had an immobilized enzymatic load twice as high as Lipozyme TL-IM, but with an activity 3-fold higher. MCI-TLL presented high initial reaction rates up to 1.0M butyric acid, while Lipozyme TL-IM showed a decrease in its activity above 0.5M. Moreover, MCI-TLL allowed a productivity of 14.5mmolg−1h−1, while Lipozyme TL-IM 3.2mmolg−1h−1, both by mass of biocatalyst.