Improved production and antioxidant activity of exopolysaccharides by submerged culture of Lentinula edodes by the addition of lignocellulose

Abstract

The objective of this study was to investigate the effect of addition of four different lignocellulose (water-treated corn straw, water-treated xylosma sawdust, alkali-treated corn straw and alkali-treated xylosma sawdust) on the production and antioxidant activity of exopolysaccharides by Lentinula edodes. To evaluate the antioxidant activity of polysaccharides, the analyses of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity, lipid peroxidation inhibition rate and erythrocyte hemolysis inhibition rate were carried out. The result clearly indicated that the exo-polysaccharide production and antioxidant activity by L.edodes mycelium was enhanced in varying degrees via the decomposition of lignocellulose. The yield of exo-polysaccharide in alkali-treated corn straw group was the highest (0.351g/L), which was 31.06% higher than that in control group after 5 days of submerged fermentation. High DPPH radical scavenging activity was observed in water-treated xylosma sawdust group, which decreased with the extension of fermentation time (23.15%-44.06%). Alkali-treated groups performed better than water-treated groups in terms of lipid peroxidation inhibitory activity. As for hemolysis inhibition activity toward erythrocytes, xylosma sawdust groups showed stronger oxidation resistance than corn straw groups.