Improved plant regeneration method of Artocarpus lakoocha Roxb. from immature seeds

Abstract

An efficient in vitro propagation method is developed for Artocarpus lakoocha Roxb. through direct shoot regeneration from cotyledonary node region of seedlings developed from immature seeds. The immature seeds were germinated on Murashige and Skoog (MS) medium having different concentrations of either 6-benzyl aminopurine (BA) or kinetin or Thidiazuron and subsequently, the cotyledonary node with primary shoot was transferred to MS medium without any growth regulator for shoot multiplication. Maximum (7.23 ± 0.46) shoots regenerated when immature seeds were cultured for 21 days on MS medium having 4.44 µM BA for germination. For rooting, shoots were pulse treated for 48 h with different concentrations of Indole-3-acetic acid (IAA) or Indole-3-butyric acid (IBA) followed by transfer on agar gelled MS basal medium. About 94.45% shoots rooted on pulse treatment with 5.0 µM IBA. Plantlets obtained after rooting were hardened and acclimatized into soil with 85–90% survival. The plantlets established into soil had similar vegetative morphology to the mother plant.