Improved PCR-RFLP for the Detection of Diminazene Resistance in Trypanosoma congolense under Field Conditions Using Filter Papers for Sample Storage

Hervé Sèna Vitouley,Burkhard Bauer,Emmanuel Allegye-Cudjoe,Zakaria Bengaly,Erick Ouma Mungube,Peter Van Den Bossche,Vincent Delespaux,Peter-Henning Clausen,Zakaria Bocoum,Oumar Diall,Thomas F Randolph,Issa Sidibe,Dirk Geysen,Boucader Diarra

doi:10.1371/journal.pntd.0001223

Abstract

Improved PCR-RFLP for the Detection of Diminazene Resistance in Trypanosoma congolense under Field Conditions Using Filter Papers for Sample Storage

Highlights

Animal African trypanosomiasis (AAT) is caused by different species of the protozoan parasite Trypanosoma and affects a wide range of domestic animals
Despite ambitious AAT control programmes such as the Pan African Tsetse and Trypanosomiasis Eradication Campaign that aim to eradicate the disease from the African continent, the control of the disease will in the foreseeable future continue to rely heavily on the use of trypanocidal drugs
Diminazene aceturate (DA) is the cheapest and most popular trypanocide. This drug, commercialized more than half a century ago, tends to become ineffective due to the spread of trypanocidal drug resistance reported in 17 African countries [2]

Summary

Introduction

Animal African trypanosomiasis (AAT) is caused by different species of the protozoan parasite Trypanosoma and affects a wide range of domestic animals. To address these important drawbacks, the Department of Animal Health of the Institute of Tropical Medicine Antwerp has been actively involved in research on trypanocidal drug resistance and in 2007 was designated Reference Centre for Livestock Trypanosomiasis, Parasite Management and Diagnosis (RCLT) by the Food and Agriculture Organization of the United Nations (FAO) and accredited to ISONORM 17025 in 2009.

Results

Conclusion