Abstract

We describe simple modifications of the HCACO experiment that include 13 C α and 13 CO constant time evolution periods. An overall duration of 28 ms involving carbon transverse magnetization in combination with an optimal magnetization-transfer efficiency results in a high sensitivity while providing reasonable resolution in the 13 C α domain. The improved protocol can be extended to pulse sequences which yield correlations between the 13 C α , 13 CO pair and the aliphatic side-chain proton resonances.

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