Abstract
Among several Azospirillum strains tested for N 2 fixation in association with wheat in a model system, 2 A. lipoferum strains (SpBr17 and 596) were found to be more efficient than the 8 other A. lipoferum and A. brasilense strains tested. Plants treated with low concentrations (0.7 μg ml −1) of 2,4-dichlorophenoxyacetic acid (2,4-D) followed by inoculation supported larger acetylene reduction activity than plants treated only with bacteria. Three broad host range plasmids carrying lacZ, as a reporter gene, controlled by nifA, nodG and ntrC promoters were transferred to A. lipoferum SpBr17 and strain 596 by conjugation. All the transconjugants showed high β-galactosidase activity in the free-living condition. Studies on colonization of wheat roots using the SpBr17 nodG- lacZ gene fusion, which expressed the lacZ gene at the highest level, indicated the localization of bacteria on the young parts of roots as a thick coat. Counts of azospirilla on plant roots showed increased association of bacteria in 2,4-D treated plants.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
