Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for Sleeping Sickness Diagnosis and Staging

Philippe Büscher,Sylvain Biéler,Vincent Jamonneau,Veerle Lejon,Wim Van Der Veken,Jacques Kaboré,Dieudonné Mumba Ngoyi,Nicolas Bebronne,Jo Robays,Daniel Eichinger

doi:10.1371/journal.pntd.0000471

Abstract

Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for Sleeping Sickness Diagnosis and Staging

Highlights

10,000 sleeping sickness patients are diagnosed and treated each year [2]
Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange centrifugation technique [7] are applied on blood. mAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al and later adapted for diagnosis of sleeping sickness and of animal infections with T
In mAECT, trypanosomes are separated from 350 ml of blood by anion exchange chromatography on diethylaminoethyl cellulose (DEAE)

Summary

Introduction

10,000 sleeping sickness patients are diagnosed and treated each year [2]. MAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al and later adapted for diagnosis of sleeping sickness and of animal infections with T.

Results

Conclusion