Abstract
AEI Aquaculture Environment Interactions Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AEI 4:251-261 (2013) - DOI: https://doi.org/10.3354/aei00087 Improved method for triacylglycerol-derived fatty acid profiling by various non-lethal and lethal sampling techniques in Atlantic salmon Rolf Erik Olsen1,*, Geir Lasse Taranger2, Terje Svåsand2, Ove Tommy Skilbrei2 1Institute of Marine Research, Matre Research Station, 5984 Matredal, Norway 2Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway *Email: rolf.erik.olsen@imr.no ABSTRACT: The current paper compares the use of adipose fin and muscle biopsies as non-lethal sampling procedures, and the suitability of triacylglycerol (TAG) versus total lipid (TOT) fatty acid composition (fatty acid methyl esters, FAME) to estimate dietary history in farmed and wild maturing Atlantic salmon Salmo salar. TAG FAME gives best resemblance to dietary history. Fatty acid composition differs between tissues, and sample series should preferably be from 1 tissue only. TOT FAME supplies informative data on dietary history in fatty tissues, but differs from TAG FAME when total lipid levels are low. The reason is a larger contribution of phospholipid FAME. In wild maturing salmon, lipid content in adipose fin and muscle is low. TAG provides good data on dietary history, while TOT is less informative. Escapees are well identified analysing 18:2n-6 and 18:3n-3 fatty acids, which are high in commercial feed. For estimating a wider range of dietary history after escape (switch to wild prey), or feed preferences in wild fish, other fatty acids have to be taken into account. This requires the use of multivariate methods, like principal component analysis. Adipose fin and muscle biopsies are good alternatives for non-lethal sampling using the TAG method. The minimum amounts of samples to be used are proposed to be 0.5 to 1 g of adipose fin and 60 to 100 mg of muscle. The method of storage (liquid nitrogen/dry ice) does not affect fatty acid compositions. Other tissues can also be used for fatty acid profiling. Snout is a good alternative, being lipid-rich even in wild fish with low muscle lipid. KEY WORDS: Fatty acid composition · Fatty acid profiling · Adipose fin · Triacylglycerol · Muscle · Snout · Escaped farmed salmon Full text in pdf format PreviousNextCite this article as: Olsen RE, Taranger GL, Svåsand T, Skilbrei OT (2013) Improved method for triacylglycerol-derived fatty acid profiling by various non-lethal and lethal sampling techniques in Atlantic salmon. Aquacult Environ Interact 4:251-261. https://doi.org/10.3354/aei00087 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AEI Vol. 4, No. 3. Online publication date: November 14, 2013 Print ISSN: 1869-215X; Online ISSN: 1869-7534 Copyright © 2013 Inter-Research.
Highlights
With the rapid expansion in farming of Atlantic salmon Salmo salar over the past 50 yr, escapes from cages have become a serious problem, both for the fish-farming industry and for the conservation of wild stocks
The biopsy samples were in the range from 40 to 100 mg, muscle samples were in the range from 400 to 900 mg, and adipose fin samples ranged from 300 to 600 mg
The lipid content and lipid class composition varied between the farmed (Sampling 1) and wild Salmo salar (Sampling 2) and between the tissue samples (Table 1)
Summary
With the rapid expansion in farming of Atlantic salmon Salmo salar over the past 50 yr, escapes from cages have become a serious problem, both for the fish-farming industry and for the conservation of wild stocks. The official figures of salmon escaping from fish farms in Norway show a variable trend ranging from 38 000 to 921 000 during the period from 2001 to 2012 (Directorate of Fisheries; www.fiskeridir.no). These figures are considered lower than the actual number of fish escaping (Baarøy et al.2004, Skilbrei & Wennevik 2006). Genetic changes have been demonstrated in wild salmon populations as a result of interbreeding between farmed escaped salmon and wild conspecifics (Crozier 1993, Clifford et al 1998, Glover et al 2013)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
