Abstract
An improved high-performance thin-layer chromatographic (HPTLC) method has been developed for quantitative analysis of stratum corneum lipids. When used for quantification of in-vivo-extracted skin lipids, the optimized method resulted in improved separation of cholesterol, cholesteryl esters, free fatty acids, and squalene. Variation in repeatability for cholesterol, cholesteryl esters and free fatty acids was under ±5%. The extraction recovery was 91.4 ± 1.2% (mean ± CV). Coefficients of variation ranged from 0.8 to 3.8%. The limits of detection S/N 3:1 and of quantification S/N 5:1 under the given conditions were: cholesteryl sulfate 12.5/25 ng, ceramide [AP]D-compound 50/250 ng, ceramide [NP] 50/125 ng, ceramide [NS] 125/250 ng, cholesterol 50/125 ng, palmitic acid 50/250 ng, and cholesteryl oleate 50/125 ng.
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