Abstract
An improved method has been developed for measuring the catalytic activity of tryptophan synthase α-subunit in cell extracts using the indole-3-glycerol phosphate (InGP) → tryptophan reaction. The method involves the chemical and enzymatic synthesis of the substrate InGP immediately before use and avoids the preparation of salt-free hydroxylamine. The method is more convenient, safer and more reliable than the traditional method employing the InGP → indole reaction.
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