Abstract
BackgroundGenomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex polysaccharide with a ramified structure. Thus, pectin- and mucilage-free extraction of DNA is a key step for further downstream PCR-based analyses.ResultsWe tested our DNA extraction method on cladode tissue (juvenile, adult, and herbaria exemplars) of 17 species of Opuntia Mill., which are characterized by a large quantity of pectin and mucilage.ConclusionWe developed a method for the extraction of gDNA free of inhibitory compounds common in species of Opuntia Mill., such as pectin and mucilage. Compared to previously extraction protocols, our method produced higher yields of high-quality genomic DNA.
Highlights
Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin
The Agarose gel electrophoresis showed the presence of large quantities of genomic DNA (gDNA) free of contaminants (Fig. 1)
The large amount of gDNA was confirmed with two different methods
Summary
Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin- and mucilage-free extraction of DNA is a key step for further down‐ stream PCR-based analyses. Especially in species with similar morphologies, can be used to characterize and differentiate species [1, 2] Such studies have used molecular techniques involving PCR amplification of DNA [3, 4] to successfully solve taxonomic and phylogenetic controversies [5]. High-quality DNA extraction is a necessary first step to conduct molecular studies This can be performed using conventional methods or commercial kits designed for particular types of samples. DNA extracted from species of cacti (Cactaceae) are often contaminated with high quantities of mucilage and pectin [10,11,12,13,14,15]
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