Abstract
Different high-performance liquid chromatography methods were examined for determinations of bergapten in natural or treated citrus oils including bergamot, lime, lemon, orange, tangerine, petitgrain and grapefruit. Comparisons among normal bonded-phase partition chromatography (Carbowax 400/Corasil), porous-layer particle adsorption chromatography (Corasil II) and totally porous microparticle adsorption chromatography (μ-Porasil and Zorbax-Sil) are discussed. In the most effective method for quantitative assay of bergapten in all the above citrus oils a Zorbax-Sil column equipped with specially constructed low dead-volume fittings was employed. A sensitivity better than 0.5 ppm for bergapten can be obtained using 254-nm ultraviolet absorbance detection.
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