Abstract

Many investigators who use cell culture as a research tool fail to give adequate attention to culture media. Virtually all “standard” nutrient media were published more than 20 years ago, and many widely used formulations are at least 30 years old. Thus, the culture medium is often the most obsolete and neglected component of the entire experimental system. This presentation describes recent improvements in cell culture media for human muscle satellite cells (HMSC) and seeks to encourage their use.

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