Abstract
Canine parvoviral enteritis remains one of the dreaded enteric viral diseases of dogs. Rapid detection of the etiological agent, Canine parvovirus-2 (CPV-2) is required for managemental intervention and initiating therapeutic and control measures. The present study describes Loop Mediated Isothermal Amplification (LAMP) using loop primers, validating the assay on all the circulating CPV-2 antigenic types and testing in 120 suspected clinical samples. The novel LAMP assay was completed within 30-45 min at 64°C in a water bath. Analytical sensitivity of the LAMP assay was 100 times more than conventional PCR and was comparable to real-time PCR. LAMP was able to detect 62 positive samples while conventional PCR could detect only in 51 samples. The developed LAMP provides a simple, rapid, cost-effective, sensitive, specific method of detection of CPV-2 infection in dogs in clinical settings.
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