Abstract
Size is the key feature of nanoparticle properties. Nanoparticle size distribution can be measured by microscopy technique and light scattering posterior to a separation technique. Discrepancies can be observed, especially in the case of polydisperse samples. Microscopy is more precise for size and shape measurement, while light scattering provides better information about concentration. An experiment is set up to investigate the feasibility of bringing comparability of Asymmetrical Flow Field-Flow Fractionation followed by Multi-Angle Light Scattering and AFM measurements. A sample made from a mixture of stock samples is built, after detailed characterisation of the stock samples. By obtaining comparability of MALS and AFM measurements, a new way to calibrate MALS instrument without the need for reference material is opened: using aliquots of the sample itself as a reference.
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