Abstract
The effects of inorganic media constituents on shoot multiplication in vitro were investigated with the Vitis hybrid ‘Remaily Seedless’. The explants were 1.5-cm sub-cultured shoot tips with 3–4 nodes. An improved medium for shoot multiplication was designed. It differs from others in its lack of added chloride and potassium iodide, and in a lower manganese sulfate concentration. The effects of KI on shoot production were also produced by MnSO 4. Iodine, a non-essential element in growth and development of higher plants, can be withheld from culture media in our conditions. The effects of manganese and KI on shoot production are discussed in relation to their involvement in auxin metabolism and transport.
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