Abstract

In the principle of lens imaging, when we project a three-dimensional object onto a photosensitive element through a convex lens, the point intersecting the focal plane can show a clear image of the photosensitive element, and the object point far away from the focal plane presents a fuzzy image point. The imaging position is considered to be clear within the limited size of the front and back of the focal plane. Otherwise, the image is considered to be fuzzy. In microscopic scenes, an electron microscope is usually used as the shooting equipment, which can basically eliminate the factors of defocus between the lens and the object. Most of the blur is caused by the shallow depth of field of the microscope, which makes the image defocused. Based on this, this paper analyzes the causes of defocusing in a video microscope and finds out that the shallow depth of field is the main reason, so we choose the corresponding deblurring method: the multi-focus image fusion method. We proposed a new multi-focus image fusion method based on sparse representation (DWT-SR). The operation burden is reduced by decomposing multiple frequency bands, and multi-channel operation is carried out by GPU parallel operation. The running time of the algorithm is further reduced. The results indicate that the DWT-SR algorithm introduced in this paper is higher in contrast and has much more details. It also solves the problem that dictionary training sparse approximation takes a long time.

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