Improved Holographic Reconstruction Procedure for Particle Field and Biological Cell Characterization

Abstract

Digital holography technique has wide range of applications in the field of science and engineering. It consists of two processes i.e. recording and reconstruction. The reconstruction procedure influences the accuracy of the measurement. The present study proposes a novel approach for the reconstruction process based on the superposition principle. The effectiveness of this proposed approach has been presented for 3D distribution of the particle field using both simulated data and experimental data. The 3D particle field has been experimentally generated using the mixture of copper particle and gelatin solution between two glass cover slip. The enhancement of resolution is subsequently achieved by implementing the lens based holographic technique. This technique is capable of providing both 3D geometrical and compositional details of crystals and biological cells etc. A single hologram provides 3D distribution and morphology of the object field.