Abstract
A compact desktop model of a high-speed counter-current chromatograph holds three identical multilayer coils in the symmetrical positions around the rotary frame to maintain perfect balance of the centrifuge system without the use of a counterweight. These multilayer coils are connected in series to make up a total capacity of 400 ml while the unique gear arrangement on the rotary frame establishes a twist-free mechanism of the flow tubes so that continuous elution can be performed without the use of rotary seal. The high performance of the present system was successfully demonstrated in separations of 10–250 mg of 2,4-dinitrophenyl amino acid mixtures in a two-phase solvent system composed of chloroform—acetic acid—0.1 M hydrochloric acid (2:2:1, v/v/v).
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