Abstract
Abstract We improved a high-performance liquid chromatographic method for the quantitative determination of ceftizoxime in human serum and urine using cefotaxime as internal standard. It employs a μ Bondapak Alkyl Phenyl column, elution with acetonitrile-phosphate buffer and measurement of UV absorption at 254 nm. Results obtained using the HPLC assay were compared to those obtained using a microbiological assay. The correlation coefficient was 0.987 (n:25). The method is rapid, accurate and reproducible with a sensitivity of 2.5 μg/ml of ceftizoxime. Cefotaxime and its major metabolite, the desacetylcefotaxime, can also be quantitated by this procedure.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
