Abstract
We report the design of a 96-well centrifugal filtration plate and its use in an automated DNA extraction method of touched objects. Cells on the touched objects such as glove prints, swabs from door handles, tools, and so on, are lysed in the 96-well filtration plate. The cell lysate is separated from the objects by centrifugation. Then automated DNA extraction and purification are carried out automatically on a robotic workstation. Such a method not only improves the DNA profile obtained from touched objects but also shortens sample processing time. Processing of 92 samples can be completed in 90 min.
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