Abstract
The gas-liquid chromatographic method previously reported by us was improved with regard to the gas chromatographic columns and their operating conditions. It became possible to perform more accurate quantification of the 22 protein amino acids, including asparagine and glutamine, in less than 25 min on a dual set of columns. At the same time, the method allowed determination of the amino acids in the range 0.5-10 μg of each by eliminating the contaminant peaks derived from solvents and reaction vials. A single sample could be analysed in an overall time of 1 h.
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