Improved functional properties of glycosylated soy protein isolate using D-glucose and xanthan gum

Abstract

Functional properties of the soy protein need to improve to have better applications in food industry. Alkali extracted and acid precipitated soy protein isolate (SPI) was glycosylated using D-glucose (G) and Xanthan gum (X) via Maillard reaction to improve solubility. The effects of SPI to G and SPI to X ratios (SPI:G = 2:1, 1:1, and 1:2; SPI:X = 100:1 and 10:1) and incubation time (0, 6, 12, and 24h) on the solubility and functional properties of glycosylated SPI were evaluated. The SPI:G ratio of 1:2 yielded a maximum degree of glycosylation of 71.1%. The solubility of SPI after glycosylation significantly increased (P < 0.05) at pH4.0-8.0 compared to SPI alone. Although the emulsion stability of glycosylated SPIshas not significantly increased (P > 0.05), the emulsifying activity improved significantly (P < 0.05). Glycosylation with SPI-X at a ratio of 10: 1 showed maximum emulsifying activity of 191.6m(2)/g (SPI alone: 66.3m(2)/g). Moreover, the SPI:X (ratio of 100:1) showed the maximum foaming activity (205mL) comparedto SPI alone (155mL). The foaming stability of SPI (2.6%) increased to 5.5 and 8.2% when using xanthan gum at the ratio of 100:1 and 10:1, respectively. Glycosylated SPI with enhanced emulsifying and foaming properties has potential to improve the functional quality of the food products.