Abstract
Fibroblasts, which play an important role in biological seal formation and maintenance, determine the long-term success of percutaneous implants. In this study, well-defined microporous structures with micropore diameters of 10–60 µm were fabricated by microelectromechanical systems and their influence on the fibroblast functionalities was observed. The results show that the microporous structures with micropore diameters of 10–60 µm did not influence the initial adherent fibroblast number; however, those with diameters of 40 and 50 µm improved the spread, actin stress fiber organization, proliferation and fibronectin secretion of the fibroblasts. The microporous structures with micropore diameters of 40–50 µm may be promising for application in the percutaneous part of an implant.
Highlights
The long-term success of the percutaneous implants relies on osseointegration and a stable soft tissue biological seal [1,2,3]
This study aims to evaluate the effect of precisely designed microporous structures manufactured by Microelectromechanical systems (MEMS) on fibroblast functionalities for possible application in the percutaneous part of an implant
We observed that the microporous structures with micropore diameters of 10–60 μm influenced the fibroblast functionalities and that those with 40 and 50 μm improved the viability, spread, actin stress fiber organization and fibronectin secretion of the fibroblasts
Summary
The long-term success of the percutaneous implants relies on osseointegration and a stable soft tissue biological seal [1,2,3]. The biological seal around the percutaneous part of an implant acts as a barrier to prevent bacterial invasion to the sub-epithelial connective tissues and the deeper area around the implant [1,4,5]. Infection and loss of biological seal are the main reasons for percutaneous implant failure [1,3,6,7,8,9,10,11]. To enable faster soft tissue biological seal establishment and good maintenance, rational surface design for the percutaneous part of implants is deemed important. Fibroblasts are believed to play an important role in biological seal formation and maintenance Soft tissue mainly consists of fibroblasts that produce extracellular matrix (ECM) and various essential components of the connective tissues, such as glycosaminoglycan and collagen [12,13].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
