Improved estimation of gross primary productivity (GPP) using solar-induced chlorophyll fluorescence (SIF) from photosystem II

Abstract

Solar-induced chlorophyll fluorescence (SIF) contains contributions from both photosystem I (PSI) and photosystem II (PSII). In theory, SIF emitted from PSII (SIFPSII) should be extracted from at-sensor SIF to quantify photosynthetic CO2 assimilation, as PSI fluorescence yield is nearly insensitive to changes in photochemical yield. In many SIF-related studies, the fraction of chlorophyll-absorbed energy allocated to PSII (β2), a key factor controlling the flux of excitation energy for SIFPSII, is simply assigned a fixed value. However, β2 is regulated in response to variations in environmental conditions to avoid an energy imbalance between PSI and PSII. By quantifying the regulating effect of the cytochrome b6f complex (Cyt b6f) on the electron transport from PSII to PSI, and its interaction with energy dissipation in both photosystems, we develop a framework to estimate β2 and PSII fluorescence yield (ΦF2), two key determinants for SIFPSII, from SIF emission, PAR, air temperature, the maximum carboxylase activity of Rubisco (Vcmax), and the maximum activity of Cyt b6f (JCB6F_max). The framework is equipped with a two-leaf scheme, enabling us to estimate SIFPSII for sunlit and shaded leaves from top-of-canopy SIF observations (SIFTOC). Our simulation results showed that β2 and ΦF2 tend to change in opposite directions with varying PAR, making the contribution of PSII to SIFTOC relatively constant. We compare gross primary productivity (GPP) mechanistically estimated from SIFPSII obtained with fixed (GPPSIF_Fβ) and dynamic β2 (GPPSIF_Dβ) against the eddy-tower-derived GPP (GPPEC) at a winter-wheat experiment site. At a half-hourly time scale, GPPSIF_Dβ is better than GPPSIF_Fβ, showing higher correlations with GPPEC (R2 = 0.74 versus R2 = 0.64 and RMSE = 6.61 μmol m−2 s−1 versus RMSE = 7.67 μmol m−2 s−1). The study provides a practical way to estimate the contribution of SIFPSII to SIFTOC, giving a better theoretical basis to SIF-based GPP estimation models.