Improved Draft Genome Sequence of a Monoteliosporic Culture of the Karnal Bunt (Tilletia indica) Pathogen of Wheat.

Anil Kumar,A K Mishra,Pallavi Mishra,Pramod W Ramteke,Vijai K Gupta,Soma S Marla,Ranjeet Maurya

doi:10.1128/genomea.00015-18

Abstract

ABSTRACTKarnal bunt of wheat is an internationally quarantined fungal pathogen disease caused by Tilletia indica and affects the international commercial seed trade of wheat. We announce here the first improved draft genome assembly of a monoteliosporic culture of the Tilletia indica fungus, consisting of 787 scaffolds with an approximate total genome size of 31.83 Mbp, which is more accurate and near to complete than the previous version.

Highlights

Karnal bunt of wheat is an internationally quarantined fungal pathogen disease caused by Tilletia indica and affects the international commercial seed trade of wheat
The Illumina and PacBio sequence reads of the TiK (Tilletia indica Karnal) isolate [3] were quality checked using FastQC version 0.11.5 [7] and fastQValidator version 0.1.1 [8], and the reads were repaired using BBmap version 37.66 [9]
Genome assembly gap filling and polishing on the merged assembly were done by GapFiller version 1.10 [13, 14] and Pilon version 1.22 [15], respectively

Summary

Introduction

Karnal bunt of wheat is an internationally quarantined fungal pathogen disease caused by Tilletia indica and affects the international commercial seed trade of wheat. The Illumina and PacBio sequence reads of the TiK (Tilletia indica Karnal) isolate [3] were quality checked using FastQC version 0.11.5 [7] and fastQValidator version 0.1.1 [8], and the reads were repaired using BBmap version 37.66 [9]. Adapter sequences along with low-quality bases were removed using PRINSEQ version 0.20.4 [10].

Results

Conclusion