Abstract
Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p < 0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth + 50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.
Highlights
Arcobacter-like species are found associated with many matrices, including shellfish in marine environments
The genus Arcobacter was separated from the genus Campylobacter in 1991 by Vandamme et al.[1] and rapidly enlarged with the description of many new species, the majority of them described from shellfish, embracing in 2017 a total of 27 species
Physical appearance of enrichment broth media supplemented with oyster homogenate or lyophilized oyster
Summary
Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. For the purpose of this study, we will mainly either use the original species names or refer to all the species as Arcobacter-like species These species are fastidious organisms and were differentiated from the campylobacters due to their ability for growth in the presence of oxygen and at significantly lower temperatures i.e. 15–30 °C3,4. Despite their diversity and isolation from different environments, Arcobacter-like species are considered zoonotic and enteropathogenic, since they have been isolated during several infections and disease episodes both in humans and in animals[36]. The prevalence rate of Arcobacterlike spp. from food products, from highest to lowest, is from poultry, seafood, pork meat, dairy products, lamb, and beef followed by rabbit[43,44]
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