Improved Cell Culture Surface for Biotechnological Research

Abstract

Cell-based assays play an important role in life science and drug discovery. Recent advances in high-throughput (HTS) and high-content screening (HCS) have evolved broad attention in the utilization of living cells as an attractive adjunct to biochemical analysis (Nichols et al. 2007). Due to the various applications the requirements for the corresponding cell cultivation surfaces are as comprehensive. Therefore Greiner Bio-One has developed the novel, non-biological Advanced TC™ polymer modification for the propagation of fastidious cells. Based on the innovative technique the cell culture surface is modified in such a way to positively influence cellular features and functions. The Advanced TC™ surface leads to consistent and homogenous cell attachment increasing the overall cell yield and reducing cell loss during washing steps. Higher proliferation rates improve cell expansion, facilitating in particular cultivation of sensitive and primary cells as well as cells cultivated under restricted growth conditions. In contrast to biological coatings the Advanced TC™ technology exhibit distinct advantages: As a result of the manufacturing process the chemical modification of the polymer assures constant product quality, facilitates sterilization of the product and reduces the risk of possible contamination. In summary Advanced TC can optimize security of cell culture as well as improve cell based screening approaches.