Abstract
Low implantation rates and high multiple pregnancy rates have been the impetus for blastocyst embryo transfer. The availability of commercially developed sequential embryo culture media has facilitated the transition from day 3 to day 5 embryo transfers. After several groups reported excellent blastocyst development with these sequential media, we began using G1/G2 (period I, Scandinavian IVF Science). Conversion of embryos from day 3 cleaved to day 5 blastocyst was lower than expected. As a result, we switched to P1/Blastocyst (period II, Irvine Scientific). Blastocyst conversion improved; however, overall embryo cleavage and day 3 pregnancy rates declined. During period III embryos were cultured in G1 for the first 72 hours and then moved to Blastocyst Medium for extended culture. The purpose of this study was to compare conversion of day 3 cleaved embryos and pregnancy rates with extended culture in different growth media combinations.
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