Abstract
In this study, a water-miscible ‘classic’ ionic liquid (IL), 1-ethyl-3-methylimidazoliumacetate ([EMIM][Ac]), has been used for lipid extraction from marine diatoms Thalassiosira lundiana CSIR-CSMCRI 001 by following a non-polar solvent partition method. The composition of lipid was determined using gas chromatography-mass spectrometry (GC-MS). In total, 91.4 mg g−1 (dry wt) of lipid was produced, out of which the percentage of docosahexaenoic acids (DHA), myristic acid, palmitic acid, and arachidonic acid was 19.6%, 15.1%, 11.2%, and 10.4%, respectively. The IL-inseparable residual waste solution was directly used to generate green fluorescent carbon dots (FCDs) by constructing a colloidal solution with the help of a surface-active IL, choline dioctyl sulfosuccinate ([Cho][AOT]). The stability of colloidal FCDs was examined using FTIR, FT-NMR, and Raman spectroscopy. FCDs were extracted from the colloidal solutions via the demicellization process and characterized using HR-TEM (2 to 5 nm) and PXRD techniques. The optical properties of colloidal FCDs were measured using UV-Vis and fluorescence spectroscopy and showed a wide range of emission (λ460 nm to λ590 nm). Such FCD stabilized colloidal solutions could be effectively used in fluorescence imaging of yeast cells, thus making the biorefinery approach more sustainable.
Highlights
The International Energy Agency (IEA) has reported that over the ve years, renewable energy capacity will be increased and exceed the possible existing energy sources, including natural gas and coal, etc
fluorescent carbon dots (FCDs) were extracted from the colloidal solutions via the demicellization process and characterized using HR-Transmission electron microscopy (TEM) (2 to 5 nm) and Powder X-ray diffraction (PXRD) techniques
The optical properties of colloidal FCDs were measured using UV-Vis and fluorescence spectroscopy and showed a wide range of emission (l460 nm to l590 nm). Such FCD stabilized colloidal solutions could be effectively used in fluorescence imaging of yeast cells, making the biorefinery approach more sustainable
Summary
The International Energy Agency (IEA) has reported that over the ve years, renewable energy capacity will be increased and exceed the possible existing energy sources, including natural gas and coal, etc. Researchers found that marine biomass such as macroalgae and microalgae are some of the best alternatives towards nding such renewable energy sources. A group of eukaryotic microalgae found in the phytoplankton (Bacillariophyta) of the world's oceans and freshwater, are believed to be responsible for about 1/5 of the primary biomass productivity on earth.[1,2] Diatoms can x about 20–25% of atmospheric carbon through photosynthesis in the presence of sunlight.[3] The shell of the diatoms is made of amorphous hydrated silica called frustules. The biological control of unique frustule morphology (bio-silici cation) has PUFAs are long-chain fatty acids that contain more than two double bonds. The most common n6 PUFAs fatty acids such as linoleic acid (18 : 2), g-linoleic acid (GLA, 18 : 3), and arachidonic acids (AA, 20 : 4) are involved in membrane synthesis
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