Improved biomedical bioactivity of polyvinyl alcohol/polyethylene oxide composite system-based nanofiber membranes via incorporating Antarctic krill protein

Abstract

Polyvinyl alcohol (PVA) and polyethylene oxide (PEO) nanofiber membranes with high porosity and specific surface area supply a suitable structural environment for cell growth, however the difficulty of cells adhesion to and growth has tremendously limited their application in biomedical materials. In this work, by incorporating Antarctic krill protein (AKP), the problem in cells adhesion and growth is successfully addressed for PVA/PEO nanofiber membranes. Meanwhile, it is worth noting that the problem that AKP is difficult to prepare nanofibers by electrospinning process is also effectively avoided. The intermolecular interactions ensure the uniform dispersion of AKP in the nanofiber membranes. And both the crystal structure content of nanofiber membranes and the ordered secondary structure content of AKP reach the maximum values of 37.41 % and 52.17 % at the AKP content of 20 %. The tensile strength is improved by 13.46 % notably. More importantly, the introduction of AKP enhances cell proliferation and viability and adds more recognition sites for cell attachment, solving the problem of difficult cell adhesion and growth. Thus, as-prepared nanofiber membranes containing AKP can supply suitable structural environment and excellent biocompatibility, for cell growth and reproduction. It is expected to be an essential candidate in biomedical materials with potential application prospects.