Abstract
Polyvinyl alcohol (PVA) is a synthetic polymer that is difficult to degrade in nature. In this study, we synthesized PVA-degrading enzymes (PVAase)-Cu3(PO4)2 hybrid nanoflowers by using crude PVAase from Bacillus niacini for PVA degradation. The influences of PVAase concentration, Cu2+ concentration, and incubation time on the nucleation and activity of the PVAase hybrid nanoflower were investigated. The maximal activity recovery of the PVAase hybrid nanoflower was approximately 85% at 0.25 mg/mL of PVAase, 0.36mM Cu2+, and 72 h incubation time. The optimum temperature and pH of PVAase did not change before and after immobilization. Compared with free PVAase, the PVAase hybrid nanoflower showed high thermal stability and storage stability. Additionally, the PVAase hybrid nanoflower displayed excellent reusability after eight cycles and promising PVA degradability, indicating its potential application in PVA degradation.
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