Abstract
Fusarium oxysporum is a pathogenic fungus that infects hundreds of plant species. This paper reports the improved genome assembly of a reference strain, F. oxysporum f. sp. lycopersici Fol4287, a tomato pathogen.
Highlights
Fusarium oxysporum is a pathogenic fungus that infects hundreds of plant species
The initial assembly was generated via SPAdes version 3.9.1 [7], combining raw Illumina and PacBio reads with default parameters
Quiver in SMRT Analysis [8] was used to polish the assembly based on the PacBio reads
Summary
Fusarium oxysporum is a pathogenic fungus that infects hundreds of plant species. This paper reports the improved genome assembly of a reference strain, F. oxysporum f. sp. lycopersici Fol4287, a tomato pathogen. The initial assembly was generated via SPAdes version 3.9.1 [7], combining raw Illumina and PacBio reads with default parameters. Quiver in SMRT Analysis (version 2.2.0) [8] was used to polish the assembly based on the PacBio reads. Further polishing was performed by mapping the Illumina reads to the assembly using BWA version 0.7.12 [9].
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