Abstract
A computer-coupled HPLC system with a refractive index detector was developed for measuring within 30 min analysis time the substrate, dextrose, and the products, acetic acid, ethanol, acetoin, acetone, butyric acid, and butanol, produced by Clostridium acetobutylicum in the acetone-butanol fermentation. Excellent resolution for each peak was achieved by adjusting the Biorad HPX-87H column temperature to 14°C.
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