Imprinted zeolite modified carbon paste electrode as a selective potentiometric sensor for blood glucose

Abstract

Imprinted zeolite modified carbon paste electrode as a potentiometric sensor for glucose analysis in blood serum sample has been developed. In this study, the type of used zeolite was TS-1. Imprinted zeolite (IZ) was synthesized with mole ratio of glucose/Si of 0.0306. The modified electrode prepared by mixing activated carbon, imprinted zeolite, and paraffin granule with mass ratio 9:4:7 from the measurement in range of 10−5 – 10−2 M is with Nernst factor of 28.6 mV/decade, and the detection limit of 4.79 x 10-5 M. The electrode was able to respond the glucose molecules in solution quickly (<30 s), stable for more than 9 weeks (130 times usage) and selective to glucose molecules. The urea, uric acid, and creatine with various concentrations did not interfere to glucose analysis by potentiometry using the electrode. Applying of the electrode for glucose analysis in spiked blood serum samples showed that it had recovery of 91.73±3.48% (n=5), while its accuracy against spectrophotometry method as commonly used method in medical field was 90.58±4.65% (n=5). Based on its performance, potentiometry using the imprinted zeolite modified carbon paste electrode is recommended as an alternative method for routine blood glucose analysis in the medical field.