Imprinted Polymeric Beads-Based Screen-Printed Potentiometric Platforms Modified with Multi-Walled Carbon Nanotubes (MWCNTs) for Selective Recognition of Fluoxetine.

Saad S.M. Hassan,Heba M. Hashem,Abd El-Galil E. Amr,Ayman H. Kamel,E.M. Abdel Bary

doi:10.3390/nano10030572

Saad S.M. Hassan, Heba M. Hashem + Show 3 more

Open Access

https://doi.org/10.3390/nano10030572

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Abstract

Herein, we present a new validated potentiometric method for fluoxetine (FLX) drug monitoring. The method is based on the integration of molecular imprinting polymer (MIP) beads as sensory elements with modified screen-printed solid contact ion-selective electrodes (ISEs). A multi-walled carbon nanotube (MWCNT) was used as a nanomaterial for the ion-to-electron transduction process. The prepared MIP beads depend on the use of acrylamide (AAm) and ethylene glycol dimethacrylic acid (EGDMA) as a functional monomer and cross-linker, respectively. The sensor revealed a stable response with a Nernstian slope of 58.9 ± 0.2 mV/decade and a detection limit of 2.1 × 10−6 mol/L in 10 mmol/L acetate buffer of pH 4.5. The presented miniaturized sensors revealed good selectivity towards FLX over many organic and inorganic cations, as well as some additives encountered in the pharmaceutical preparations. Repeatability, reproducibility and stability have been studied to evaluate the analytical features of the presented sensors. These sensors were successfully applied for FLX assessment in different pharmaceutical formulations collected from the Egyptian local market. The obtained results agreed well with the acceptable recovery percentage and were better than those obtained by other previously reported routine methods.

Highlights

Fluoxetine (FLX) is one of the five drugs inserted under the selective serotonin re-uptake inhibitors (SSRIs) category that is used throughout the world as an anti-depressant drug
In the unwashed molecular imprinting polymer (MIP) spectrum, it can be shown that the clear peaks at 3362 and 1167 cm−1 refer to N–H stretching and C–N of the secondary amine group that present in FLX, respectively
The strong and sharp peak of cross-linked AAm monomer in MIPs and non-imprinted polymer (NIP) that appeared at 1717 cm−1 corresponds to –C=O and all peaks from 1256 to 1128 cm−1 corresponds to –C–O stretching of the polymeric backbone

Summary

Introduction

Fluoxetine (FLX) is one of the five drugs inserted under the selective serotonin re-uptake inhibitors (SSRIs) category that is used throughout the world as an anti-depressant drug. According to the world federation for mental health, depression is occurring as a reason for different problems that affect the behavior of humans. It can cause economic problems, increase the rate of unemployment and it is a main reason for family disturbance [1]. It can cause occupational stress when the worker is facing work demands and is not matched with its requirements [2].

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