Imprinted Polymer Beads Loaded with Silver Nanoparticles for Antibacterial Applications.

Weifeng Liu,Solmaz Hajizadeh,Haiyue Gong,Lei Ye

doi:10.1021/acsabm.1c00045

Weifeng Liu, Solmaz Hajizadeh + Show 2 more

Open Access

https://doi.org/10.1021/acsabm.1c00045

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Abstract

After the emergence of multidrug-resistant strains, antibiotic resistance in bacteria has become an important problem. Thus, materials for combating multidrug-resistant bacteria are of vital importance. In this work, we developed an antibacterial material that can selectively capture and destruct bacteria on the basis of their physical characteristics. To achieve bacterial capture and deactivation with a single material, we used bacterial cells as templates to synthesize surface-imprinted polymer beads in bacteria-stabilized Pickering emulsions. Acrylate-functionalized polyethylenimine was used to coat the bacterial surface so that the coated bacteria can act as a particle stabilizer to establish an oil-in-water Pickering emulsion. Hydrophobic Ag nanoparticles were introduced into the oil phase composed of cross-linking monomers. Bacteria-imprinted beads (BIB) were obtained after the oil phase was polymerized. Bacterial binding experiments confirmed the importance of the imprinted sites for specific recognition with the target bacteria. The Ag nanoparticles embedded inside the polymer beads enhanced bacterial inactivation and reduced the leakage of heavy metal in aquatic environment. The combination of bacteria-imprinting with delivery of general-purpose antibacterial reagents offers a useful approach toward selective capture and destruction of bacteria.

Highlights

With the increase of microbial contamination, removal and destruction of microbial pathogens have become an important challenge concerning human health.[1]
Substantial efforts have been dedicated to developing new antimicrobial materials such as nanozymes, metal nanoparticles, cationic polymers, and transition-metal complexes.[4−8] Among these antibacterial materials, Ag is considered an ideal candidate for inactivation of pathogenic bacteria because of its high potency and broadspectrum antimicrobial activity.[9]
We demonstrated a new approach to preparing antibacterial materials by combining molecular imprinting with Ag nanoparticles

Summary

INTRODUCTION

With the increase of microbial contamination, removal and destruction of microbial pathogens have become an important challenge concerning human health.[1]. Among the many reported antibacterial materials, Ag-containing composites have shown highly effective antimicrobial effects.[12−15] Because of their unique physical/chemical properties, engineered nanoparticles have found wide applications, such as in medicine, textiles, cosmetics, environmental engineering, agriculture, etc.[16−18] As one special type of engineered. The aim of this work was to develop a new type of antimicrobial material that can selectively capture and deactivate bacteria without imparting antibiotic resistance. To realize these functions with a single material, surfaceimprinted bacteria binding sites and antimicrobial Ag nanoparticles were integrated into stable polymer beads via Pickering emulsion polymerization. This work represents the first example of turning molecularly imprinted polymers into selective bactericide by incorporating metal nanoparticles

EXPERIMENTAL SECTION

RESULTS AND DISCUSSION

CONCLUSIONS

■ ACKNOWLEDGMENTS

■ REFERENCES