Abstract
A new simple, accurate and reasonably sensitive spectrophotometric method is described for the determination of reserpine based on the oxidation with sodium metaperiodate in the presence of acetic acid and coupling with 3-methyl-2-benzo thiazolinone hydazone hydrochloride (MBTH) to form highly blue coloured product showing maximum absorbance at 630 nm. Beer's law is obeyed in the range 0.2–1.6 µg ml−1. The relative standard deviation was found to be less than 1%. The proposed method can be applied for the estimation of reserpine in tablets with no evidence of interference from excipients.
Highlights
The pharmaceutical properties of Rauwolfia alkaloids are mainly due to the presence of indole nucleaus, which have been extensively used as activeD
A number of analytical methods have been proposed for the determination of reserpine based on voltametry [6], spectrofluorometry [7]h,igh performance liquid chromatography [8-101, gas chromatography [ II ], room temperature phosphometry [I 2,131, flow injection chemiluminescence[ I41 and polarography [ I51
Sodium metaperiodate and methyl-2-benzo thiazolinone hydazone hydrochloride (MBTH) were obtained from Merck
Summary
The pharmaceutical properties of Rauwolfia alkaloids are mainly due to the presence of indole nucleaus, which have been extensively used as active. S a h u : ingredients in the pharmaceutical preparations [I]. A number of analytical methods have been proposed for the determination of reserpine based on voltametry [6], spectrofluorometry [7]h,igh performance liquid chromatography [8-101, gas chromatography [ II ] , room temperature phosphometry [I 2,131, flow injection chemiluminescence[ I41 and polarography [ I51. The proposed method is successfully applicable for the determination of reserpine in pure or pharmaceuticalformulations with good accuracy and precision. Materials Reserpine (Himedia, India) and its pharmaceutical formulations were obtained from commercial sources. Sodium metaperiodate and MBTH were obtained from Merck. Sodium metaperiodate solution (1 X lo'* M) and MBTH (1 X 1o * M~) were prepared in double distilled water and kept in dark bottles to protect from light.
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