Abstract
MicroRNAs (miRNAs) as small RNA and post-transcriptional modulators are shown to have regulatory effects for different cellular activities and pathways, such as metabolism, virus replication and also cell growth. In addition, miRNAs can regulate the replication of the hepatitis B virus (HBV). Therefore, the expression profile of miRNAs was evaluated in HBV-infected patient groups and healthy controls. The expression levels of the following microRNAs (as noninvasive biomarkers) were compared in two experimental (those with various stages of HBV infection) and control groups to evaluate their diagnosis ability: mir141-5p and mir501-5p. RNA extraction was performed for 45 serum samples. The miRCURY LNA™ Universal RT-miRNA-PCR system and miRNA PCR panels were used for measuring microRNA expression profiles. To normalize quantitative values, the endogenous reference by UniSp6 expression was used. Serum mir141-5p and mir501-5 were significant None in patient in different stages of HBV infection(p<0.001) than in controls(p<0.01). Receiver operating characteristic (ROC) curve analyses suggested that serum has mir141-5p and mir501-5p none significant diagnostic value for HBV infection. Results suggest that mir141-5p and mir501-5 can not be used as diagnostic biomarkers for monitoring of HBV infection and other biomarkers in this disease need to be investigated.
Highlights
Studies have shown that one-third of the world’s population is influenced by Hepatitis B virus (HBV) infection, which is considered a certain health concern all over the world, of whom over 350 million people have been diagnosed with chronic hepatitis B virus (HBV) (CHB) infection [1]
Cirrhosis and hepatocellular carcinoma (HCC) known for their high mortality rate are among the complications of HBV infection [18,19]
The present study aimed at assessing the serum levels of two miRs miR-141-5p and miR-141-5p in patients infected with HBV in clinical different stages compared with the healthy control group
Summary
Studies have shown that one-third of the world’s population is influenced by Hepatitis B virus (HBV) infection, which is considered a certain health concern all over the world, of whom over 350 million people have been diagnosed with chronic HBV (CHB) infection [1]. Zhang et al were first studied miRNAs, which directly target HBV transcripts They found that HBsAg expression and HBV replication were appropriately decreased by miR-199a-3p and miR-210-5p [11]. Novel methods have emerged to use miRNAs as biomarkers for liver disease by recognition of miRNAs capable of indicating pathophysiological conditions of human bodily fluids (HBF). They are considered suitable biomarkers for early and pre-symptomatic diagnosis due to their availability, great stability in the circulation and expression patterns consistent with the tissues [16]. In this study, the expression profile of a panel of miRNAs was analyzed in sera of HBV infected patient groups and healthy controls
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