Abstract

Blastomere loss occurs in ∼50% of frozen-thawed cleavage embryos. Resumption of mitotic division after in vitro culture has proven to be a good indicator of viability of frozen cleavage stage embryos. However, blastomeres of blastocyst are small in size and volume and viability after thawing after in vitro culture is less easy to define by detecting changes in blastomere number. Re-expansion is the prerequisite for subsequent implantation of frozen blastocyst. Herein we investigated the impact of blastomere loss on subsequent blastocoele re-expansion.

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