Abstract
Male infertility is a common and complex problem affecting 1 out of 20 men. Despite extensive research in this area, in many cases, the underlying causes are unknown. Epigenetic changes control a series of processes within the body, including male fertility. Classification of infertile men using a more detailed analysis of DNA methylation patterns could reveal a new level of low rates of fertilization, implantation, or pregnancy. In this context, it seemed to us to use the techniques available to evaluate the degree of global methylation of DNA in infertile patients who have modified sperm counts, but also those who apparently do not have a clear cause of infertility. For this we used the Quest 5mC-Zymoresaerch-ELISA kit that can detect within about 5 hours the global level of genome methylation. Claims on which common illnesses have an epigenetic base are still open to speculation, but if true, it can imprint a new direction in medicine. Our data, although from a pilot study, are consistent with those in the literature. A recent study has shown that DNA methylation levels were significantly higher in oligoasthenoteratozoospermia patients than in the control group and the increase in global DNA methylation and histone retention in men with oligoasthenoteratozoospermia.
Highlights
DRAGOS BOTEZATU1,2#, CRISTINA POPESCU3, ANDREI-DAN KORODI4, CRISTIAN FURAU5, GHEORGHE FURAU5, BOGDAN SOCEA6,7*#, CRANGU IONESCU6,7#, MIHAI DIMITRIU6,7#, DIANA CLAUDIA GHEORGHIU7,8#, NICOLAE GHEORGHIU7,8#, IOIART IOAN1# 1Vasile Goldis Western University of Arad, Faculty of Medicine, Department of Urology, 86 Liviu Rebreanu Str., 310414, Arad, Romania 2Department of Urology, Arad County Emergency Hospital, 86 Liviu Rebreanu Str., 310414, Arad, Romania 3Vasile Goldis Western University of Arad, Department of Experimental and Applied Biology, Institute of Life Sciences, 86 L.iviu Rebreanu Str., 310414, Arad, Romania 4Vasile Goldis Western University of Arad, Faculty of Medicine, Department of Surgery, 86 L.iviu Rebreanu Str., 310414, Arad, Romania 5Vasile Goldis Western University of Arad, Faculty of Medicine, Department of Obstetrics and Gynecology, 86 Liviu Rebreanu Str., 310414, Arad, Romania 6Sf
If feminine causes have benefited from much more intense and prolonged studies, and multiple causes have been identified from congenital malformations [4], ovulatory disorders [5,6,7], polycystic ovary syndrome [8,9], thrombophilia [10,11,12], malignant pathology [13,14], endocrine disorders, the causes of male infertility still remain unclear in high percentage and with multiple correlations with pathologies that can lead to erectile dysfunction of the cardiovascular cause [15]
Experimental part Material and method. In this context we used the techniques available to evaluate the degree of global methylation of DNA in infertile patients who have modified sperm counts, and those who apparently do not have a clear cause of infertility
Summary
In this context we used the techniques available to evaluate the degree of global methylation of DNA in infertile patients who have modified sperm counts, and those who apparently do not have a clear cause of infertility. For this we used the Quest 5mC-Zymoresaerch-ELISA kit that can detect within about 5 h the global level of genome methylation. The mean values, analysis and calculation of the percentage of methylation are shown below These values were compared with the standard curve obtained from the test kit control samples (table 1, fig.).
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