Abstract
We report studies of the import into mitochondria of MTF1, a nucleus-encoded factor that markedly stimulates the specific transcription of mitochondrial DNA. Unlike most of the mitochondrial precursor proteins studied so far, import of MTF1 does not appear to require a receptor on the outer membrane, membrane potential across the inner membrane, or ATP hydrolysis. Its import is not affected by low temperature. It lacks a cleavable presequence but translocates across the inner membrane through its amino terminus; its sorting is independent of hsp60. Our results indicate an unusual and distinct import pathway for MTF1 into the yeast mitochondria.
Highlights
We report studies of the import into mitochondria of MTFl, a nucleus-encoded factor that markedly stimulates the specific transcription of mitochondrial DNA
We report that the import ofMTF1, a nucleusencoded mitochondrial protein, takes place through an unusual pathway, which does not require the receptor on the outer mitochondrial membrane, the membrane potential across the inner membrane, and ATP hydrolysis
We have investigated the import of the MTF1 gene product into isolated mitochondria
Summary
Vol 270, No 20, Issue of May 19, pp. 11970-11976, 1995 Printed in U.S.A. Import of Transcription Factor MTFl into the Yeast Mitochondria Takes Place through an Unusual Pathway*. Unlike most of the mitochondrial precursor proteins studied so far, import of MTFl does not appear to require a receptor on the outer membrane, membrane potential across the inner membrane, or ATP hydrolysis. Our results indicate an unusual and distinct import pathway for MTFl into the yeast mitochondria Mitochondria have their own genome [1], which is transcribed by a machinery that consists of a core polymerase [2] and a mitochondrial transcription factor MTF1 [3], both of which are encoded in the nucleus and are imported into the mitochondria from the cytoplasm. We report that the import ofMTF1, a nucleusencoded mitochondrial protein, takes place through an unusual pathway, which does not require the receptor on the outer mitochondrial membrane, the membrane potential across the inner membrane, and ATP hydrolysis.
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