Abstract
Background We previously showed that plasmacytoid dendritic cells (pDCs) derived from females can produce significantly more IFN-alpha in response to HIV-1 and HIV-1-encoded TLR7/8 ligands than pDCs derived from males, resulting in stronger secondary activation of CD8+ T cells (Meier et al., Nat Med 2009). Given the crucial role of interferon regulatory factor 7 (IRF7) in the regulation of type I IFN production by pDCs, the goal of the current study was to investigate its impact on the observed differences.
Highlights
We previously showed that plasmacytoid dendritic cells derived from females can produce significantly more IFN-alpha in response to HIV-1 and HIV-1-encoded TLR7/8 ligands than pDCs derived from males, resulting in stronger secondary activation of CD8+ T cells (Meier et al, Nat Med 2009)
Given the crucial role of interferon regulatory factor 7 (IRF7) in the regulation of type I IFN production by pDCs, the goal of the current study was to investigate its impact on the observed differences
Phosphorylation levels of proteins involved in the TLR7 pathway including IRF7 were measured in pDCs by phospho-flow cytometry at baseline and at different time-points after TLR7 stimulation
Summary
We previously showed that plasmacytoid dendritic cells (pDCs) derived from females can produce significantly more IFN-alpha in response to HIV-1 and HIV-1-encoded TLR7/8 ligands than pDCs derived from males, resulting in stronger secondary activation of CD8+ T cells (Meier et al, Nat Med 2009). Given the crucial role of interferon regulatory factor 7 (IRF7) in the regulation of type I IFN production by pDCs, the goal of the current study was to investigate its impact on the observed differences
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