Abstract
Introgression between related species with different ploidy levels has played important roles in wheat subspecies differentiation. Persian wheat, a cultivated tetraploid wheat subspecies (Triticum turgidum subsp. carthlicum), is postulated to have evolved through interploidy hybridization between tetraploid and hexaploid wheats. Here, we report evidence for the origin of subsp. carthlicum based on the discovery of a new allele for the 5th-to-6th exon region of the Wknox1bKNOTTED1-type homeobox gene in a common wheat subspecies (T. aestivum subsp. carthlicoides). In this Wknox1b region, subsp. carthlicoides contains an inverted duplication mutation in the 3' flanking region of a 157-bp MITE insertion site. This structural mutation resulted in the suppression of Wknox1b expression in subsp. carthlicoides, but no structural mutation was observed in the same region of subsp. carthlicum. In addition, the carthlicum allele for the Wknox1b 5th-to-6th exon region exhibited the same sequence as that in the wild emmer wheat subsp. dicoccoides. These observations support an alternative hypothesis that subsp. carthlicum evolved by interploidy hybridization between subsp. carthlicoides and tetraploid wheat.
Highlights
Polyploid wheat species evolved through natural hybridization and allopolyploidization, and the subsequent interploidy introgression between tetraploid and hexaploid wheat species was at least partly associated with subspecies diversification (Matsuoka, 2011)
Subspecies carthlicum was proposed to have originated from spontaneous hybridization between subsp. carthlicoides and cultivated emmer wheat, T. turgidum subsp. dicoccon (Schrank) Thell. (Kuckuck, 1979)
A wheat ortholog of maize knotted1 and rice OSH1, Wknox1, belongs to the class I KN1-type homeobox (KNOX) gene family on the homoeologous group 4 chromosomes (Takumi et al, 2000), which function in main
Summary
Polyploid wheat species evolved through natural hybridization and allopolyploidization, and the subsequent interploidy introgression between tetraploid and hexaploid wheat species was at least partly associated with subspecies diversification (Matsuoka, 2011). Cytological and molecular distinction of the two subspecies carthlicum and carthlicoides Ten tetraploid accessions of T. turgidum (AABB) subsp. The MITE-containing band was missing in tetraploid wheat accessions including all those examined of subsp. In addition to CS and Ldn, 10 accessions of wild tetraploid wheat, T. turgidum subsp.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
